Fused in sarcoma undergoes cold denaturation: Implications on phase separation
Por um escritor misterioso
Descrição
The mediation of fused in sarcoma (FUS) protein liquid-liquid phase separation (LLPS) is generally attributed to the low-complexity and disordered domains, while the role of its folded domains remains unknown. In this work we questioned the role of the folded domains on the full-length (FL) FUS LLPS and studied the influence of several metabolites, ions and overall conditions on the LLPS process using turbidity assays, differential interference contrast microscopy and nuclear magnetic resonance spectroscopy. We demonstrate that FL FUS LLPS is highly responsive to the surrounding conditions, and that overall intrinsic disorder is crucial for LLPS. To promote such disorder, we reveal that the FUS RNA-recognition domain (RRM) and the zinc-finger motif (ZnF) undergo cold denaturation above 0ºC, at a temperature that is determined by the conformational stability of the ZnF domain. We hypothesize that, in cold shock conditions, cold denaturation might provide a pathway that exposes additional residues to promote FUS self-assembly. Such findings mark the first evidence that FUS globular domains may have an active role in stress granule formation in cold stress.
Temperature-Controlled Liquid–Liquid Phase Separation of Disordered Proteins
Biomolecules, Free Full-Text
Rapid disassembly of SGs upon recovery from cold shock in S.
IJMS, Free Full-Text
The differential response of FUS to cellular stress. Cells expressing
Liquid–Liquid Phase Separation and Its Mechanistic Role in Pathological Protein Aggregation - ScienceDirect
Increase in divalent cation concentration leads to dissolution of
Plot of the characteristic length determined by USAXS as a function of
PDF] Fused in Sarcoma: Properties, Self-Assembly and Correlation with Neurodegenerative Diseases
Fused in sarcoma undergoes cold denaturation: Implications on phase separation
Biomolecules, Free Full-Text
Phase separation drives tumor pathogenesis and evolution: all roads lead to Rome
Coarse grain model of cold denaturation. a Free energy difference
de
por adulto (o preço varia de acordo com o tamanho do grupo)
