Turbo FISH: A Method for Rapid Single Molecule RNA FISH
Por um escritor misterioso
Descrição
Advances in RNA fluorescence in situ hybridization (RNA FISH) have allowed practitioners to detect individual RNA molecules in single cells via fluorescence microscopy, enabling highly accurate and sensitive quantification of gene expression. However, current methods typically employ hybridization times on the order of 2–16 hours, limiting its potential in applications like rapid diagnostics. We present here a set of conditions for RNA FISH (dubbed Turbo RNA FISH) that allow us to make accurate measurements with no more than 5 minutes of hybridization time and 3 minutes of washing, and show that hybridization times can go as low as 30 seconds while still producing quantifiable images. We further show that rapid hybridization is compatible with our recently developed iceFISH and SNP FISH variants of RNA FISH that enable chromosome and single base discrimination, respectively. Our method is simple and cost effective, and has the potential to dramatically increase the throughput and realm of applicability of RNA FISH.
Amplified Fluorescence in Situ Hybridization by Small and Bright Dye-Loaded Polymeric Nanoparticles
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Papers — Syd Shaffer Lab
Design, Labeling, and Application of Probes for RNA smFISH
Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response - ScienceDirect
Paired Capture and FISH Detection of Individual Virions Enable Cell-Free Determination of Infectious Titers
PDF] Real-Time Monitoring of Fluorescence in Situ Hybridization Kinetics.
Genome oligopaint via local denaturation fluorescence in situ hybridization - ScienceDirect
Single-molecule FISH in Drosophila muscle reveals location dependent mRNA composition of megaRNPs
Enzyme-based synthesis of single molecule RNA FISH probes
Quantitative gene expression analysis in Caenorhabditis elegans using single molecule RNA FISH.
Methods for spatial and temporal imaging of the different steps involved in RNA processing at single‐molecule resolution - Markey - 2021 - WIREs RNA - Wiley Online Library
Methods for spatial and temporal imaging of the different steps involved in RNA processing at single‐molecule resolution - Markey - 2021 - WIREs RNA - Wiley Online Library
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